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Culture of Animal Cells: A Manual of Basic Techniques

By: Freshney, R.IanPublication details: New Jersey John Wiley & Sons, Inc., Publication 2005 Edition: 5ISBN: 9812532234Subject(s): ZoologyDDC classification: R 591.0724 Summary: 1. Introduction 2. Training Programs 3. Biology of Cultured Cells 4. Laboratory Design and Layout 5. Equipment 6.Aseptic Technique 7. Safety, Bioethics and Validation 8. Culture Vessels and Substrates 9. Defined Media and Supplements 10. Serum-Free Media 11. Preparation and Serilization 12. Primary Culture 13. Subculture and Cell lines 14. Cloning and Selection 15. Cell Separation 16. Characterization 17. Differentiation 18. Transformation and Immortalization 19. Contamination 20.Cryopreservation 21. Quantitation 22. Cytotoxicity 23. Culture of specific cell types 24. Culture of Tumor cells 25. Organotypic culture 26. Scale-up 27. Specialized Techniques 28. Problem Solving 29. In Conclusion
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Holdings
Item type Home library Call number Materials specified Status Date due Barcode
REF REF Parvatibai Chowgule College of Arts and Science, Margao
Reference
R 591.0724 FRE/Cul (Browse shelf(Opens below)) Not for loan PCC- 39536

1. Introduction
2. Training Programs
3. Biology of Cultured Cells
4. Laboratory Design and Layout
5. Equipment
6.Aseptic Technique
7. Safety, Bioethics and Validation
8. Culture Vessels and Substrates
9. Defined Media and Supplements
10. Serum-Free Media
11. Preparation and Serilization
12. Primary Culture
13. Subculture and Cell lines
14. Cloning and Selection
15. Cell Separation
16. Characterization
17. Differentiation
18. Transformation and Immortalization
19. Contamination
20.Cryopreservation
21. Quantitation
22. Cytotoxicity
23. Culture of specific cell types
24. Culture of Tumor cells
25. Organotypic culture
26. Scale-up
27. Specialized Techniques
28. Problem Solving
29. In Conclusion

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