TY - BOOK AU - Nagar, Santosh and Adhav, Madhavi TI - Practical Biotechnology and Plant Tissue Culture SN - 81-219-3200-9 U1 - 581.88.072 PY - 2009/// CY - Delhi PB - S. Chand & Company KW - Biotechnology N2 - Contents 1. Principle, working and uses of instruments: Autoclave, Hot air oven, Incubator, Laminar air flow, pH meter. Spectrophotometer, Colarimeter, Centrifuge, Ultracentrifuge, Paper chromatography, Thin Layer chromatography, Paper electrophoresis, Gel electrophoresis 2. Preparation of culture media 3. Preparation of standard solution 4. Isolation of bacteria by streak plant method 5. Isolation of bacteria by pour plate method 6. To determine the growth characteristics of E.coli using plating technique 7. Growth characteristics of E.coli using turbidimetric method 8. Isolation of plasmid from E.coli by alkali lysis method and to analyze plasmid DNA by agarose gel electrophoresis 9. Isolation of bacteria genomic DNA from E.coli or Serratia marsences 10. To determine the DNA molecular size 11. Purification of DNA Molecular size 12. To perform Bacteriophage titration 13. To perform restriction digestion of DNA with enzyme viz. Eco RI and Hind III and to analyze the restriction pattern by agarose gel electrophoresis 14. To perform transformation of the given bacterial population 15. DNA amplification by Polymerase chain reaction 16. Preparation of Plant Tissue culture media Preparation of Explant and its sterilization 17. To isolate protoplast from the plant tissue 18. Viability testing of protoplast 19. Demonstration of protoplast fusion using polyethylene glycol 20. Demonstration of Callus culture technique 21. Initiation of callus from radical tip of Phaseolus vulgaris 22. Demonstration of cell suspension culture 23. Demonstration of Androgenesis is Dature 24. Demonstration of Somatic embrogenesis 25. Demonstration of Organogenesis 26. Estimation of Growth Index 27. Preparation of Artificial seeds 28. Transfer of Plantlets to green house condition/field 29. Isolation of Genomic DNA from plant cells 30. Isolation of Agrobacterium sps. From soil & study of growth characteristics 31. Isolation of Agro bacterium sps. From crown gall 32. Demonstration of b-glucuronidase (gus) activity by histrochemical assay 33. Demonstration of DNA polymorphism through Restriction Fragment Length Polymorphism (RFLP) of different crop varieties 34. Demonstration of DNA polymorphism through Random Amplified Polymorphism DNA (RAPD) ER -