Laboratory Manual on Seed Pathology

seed pathology,inspection of dry seed sample, detection of seed-borne Fungi, examination od seed-borne fungi, blotter method, agar plate method, deep freezing blotter method, 2, 4-D method, rolled paper towel method, comparison of incubation method, to detect fungi associated with different categories of seed, growing on test, study of seedling symptom test in test tube, study of efficacy of fungicide in controlling seed borne pathogens, efficacy of fungicide against certain fngi with heavy inoculum pressure, comparison of dry seed treatment and wet seed treatment, find the exact location of infection component plating, whole mount method, microtome sectioning, to detect ustilago nuda tritici (loose smut) in wheat grain, to detect ephhelis oryzae (udbatta) in rice seed, to study the mode of transmission of fusarium oxysporum f. sp. udum (wilt of pigeonpea), to test vegetatively propagated plant parts, detection of seed- borne obligated parasite, to determine the severity of sed infection, effect of fungicide and rhizobium seed treatment on nodulation in chickpea, effect of temperature on infection percentage of bipoloris oryzae (brown spot of paddy), detection of seed borne bacteria, detection of seed borne bacteria by visual seperation, detection of xanthomonas campestris pv. compestris (black rot of cabbage) in seeds of cabbage, moist paper technique, quartz sand technique, water agar media method, xanthomonas campestris, pseudomonas, pseudomonas avenae, pseudomonas fuscovaginae, xanthomonas campestris, malvacearum, Detection of seed borne bacteria by dot immunobinding assay (DIBA), pseudomonas solanacearum, mosiac virus in bean seeds, ELISA test, anguina tritici, ear cockle, aphelenchoides besseyi, to detect nematodes associated with the rhizomes