Gene Biotechnology

1. From Classical Genetics to Modern Genetics
2. Genetic Engineering and r-DNA Technology
3. Genetic Complexity
4. Enzymes used in Gene Manipulation of DNA and RNA
5. Restriction Endonucleases-Molecular Scalpes
6. Isolation and Purification of DNA
7. Plasmids
8. Isolation and Purification of Plasmid DNA
9. Preparation of RNA
10. Autoradiography
11. Electrophoresis of DNA
12. Pulsed Field Gel Electrophoresis (PFGE)
13. Nucleic Acid Staining
14. Nucleic Acid Labelling
15. Molecular Probes
16. Hybridization Techniques
17. DNA Fingerprinting (DNA Profiling )
18. Restriction Fragment Length Polymorphism
19. Blotting Techniques
20. Vectors
21. Gene Cloning
22. Screening of Recombinants
23. Protoplast Technology – Fusion Techniques
24. DNA-Protein Interactions
25. DNA Libraries – (a) Genomic Library (b) cDNA Library
26. Chromosome Walking and Jumping
27. Site-Directed Mutagenesis
28. DNA Synthesis
29. Techniques of (Chromosome) Gene Mapping
30. DNA Sequencing (Gene Sequencing)
31. Ribozymes
32. Polymerase Chain Reaction (PCR)
33. Random Amplification of Polymorphic DNA (RAPD)
34. Subtraction Hybridization
35. Ribotyping
36. Protein Engineering
37. Strain Improvement Of Industrially Important Organism
38. Gene Transfer Technologies (DNA Transfer Methods)
39. Transgenic Animals
40. Human Gene Therapy
41. Gene Targeting
42. Antisense Therapy
43. RNA Interference
44. Flow Cytogenetics
45. Human Genome Project – Part I
46. Human Genome Project – Part II
47. Genomics
48. Proteomics
49. Bioinformatics – I (Primer)
50. Bioinformatics – II (Tools and Techniques)
51. Ethical, Legal, Social, Environmental and Health Issues Related to Gene Biotechnology
52. Synthetic Biology