Contents<br/>1. Principle, working and uses of instruments: Autoclave, Hot air oven, Incubator, Laminar air flow, pH meter. Spectrophotometer, Colarimeter, Centrifuge, Ultracentrifuge, Paper chromatography, Thin Layer chromatography, Paper electrophoresis, Gel electrophoresis<br/>2. Preparation of culture media<br/>3. Preparation of standard solution<br/>4. Isolation of bacteria by streak plant method<br/>5. Isolation of bacteria by pour plate method <br/>6. To determine the growth characteristics of E.coli using plating technique<br/>7. Growth characteristics of E.coli using turbidimetric method<br/>8. Isolation of plasmid from E.coli by alkali lysis method and to analyze plasmid DNA by agarose gel electrophoresis <br/>9. Isolation of bacteria genomic DNA from E.coli or Serratia marsences<br/>10. To determine the DNA molecular size<br/>11. Purification of DNA Molecular size<br/>12. To perform Bacteriophage titration <br/>13. To perform restriction digestion of DNA with enzyme viz. Eco RI and Hind III and to analyze the restriction pattern by agarose gel electrophoresis<br/>14. To perform transformation of the given bacterial population<br/>15. DNA amplification by Polymerase chain reaction<br/>16. Preparation of Plant Tissue culture media<br/>Preparation of Explant and its sterilization<br/>17. To isolate protoplast from the plant tissue <br/>18. Viability testing of protoplast<br/>19. Demonstration of protoplast fusion using polyethylene glycol<br/>20. Demonstration of Callus culture technique<br/>21. Initiation of callus from radical tip of Phaseolus vulgaris<br/>22. Demonstration of cell suspension culture<br/>23. Demonstration of Androgenesis is Dature<br/>24. Demonstration of Somatic embrogenesis<br/>25. Demonstration of Organogenesis<br/>26. Estimation of Growth Index<br/>27. Preparation of Artificial seeds<br/>28. Transfer of Plantlets to green house condition/field<br/>29. Isolation of Genomic DNA from plant cells<br/>30. Isolation of Agrobacterium sps. From soil & study of growth characteristics<br/>31. Isolation of Agro bacterium sps. From crown gall <br/>32. Demonstration of b-glucuronidase (gus) activity by histrochemical assay<br/>33. Demonstration of DNA polymorphism through Restriction Fragment Length Polymorphism (RFLP) of different crop varieties<br/>34. Demonstration of DNA polymorphism through Random Amplified Polymorphism DNA (RAPD)<br/><br/>
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Biotechnology
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Parvatibai Chowgule College of Arts and Science, Margao
Parvatibai Chowgule College of Arts and Science, Margao